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xtt reagent  (Biotium)


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    Structured Review

    Biotium xtt reagent
    Xtt Reagent, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xtt+reagent/XTT+Cell+Viability+Assay+Kit/10__3390_slash_jox16020065-50-0-7
    Average 95 stars, based on 166 article reviews
    xtt reagent - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    XTT Assay:

    Article Title: Hexosamine Biosynthesis Is a Possible Mechanism Underlying Hypoxia’s Effects on Lipid Metabolism in Human Adipocytes
    Article Snippet: .. XTT assay: XTT reagent (Biotium Inc, Catalog #30007) was added directly to culture media per manufacturer’s instructions and incubated for 2 hours at 37°C. ..

    Incubation:

    Article Title: Hexosamine Biosynthesis Is a Possible Mechanism Underlying Hypoxia’s Effects on Lipid Metabolism in Human Adipocytes
    Article Snippet: .. XTT assay: XTT reagent (Biotium Inc, Catalog #30007) was added directly to culture media per manufacturer’s instructions and incubated for 2 hours at 37°C. ..

    Article Title: Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer
    Article Snippet: Densitometry was performed using an Odyssey Infrared Imaging System and software (LI-COR Biosciences Inc., Lincoln, NE, USA); densitometry signals for glutamine synthetase, glutaminase, and glutamate dehydrogenase were normalized to actin densitometry levels. .. Semipermeable transwell membrane inserts (0.4 μm, Corning Costar Inc., Tewksbury, MA, USA) containing 15,000 3T3L1 preadipocytes or mature adipocytes were placed above 2000 PanIN/PDAC cells in 24-well culture plates in 500 μl DMEM, 0.5%FCS with glucose/glutamine concentrations as indicated, cultured 72 h, transwells removed, and 50 μl XTT reagent (Biotium Inc., Hayward, CA, USA) added, cells incubated overnight, then 100 μl of media was removed and added to a 96-well assay plate (Corning Costar Inc., Tewksbury MA, USA, Cat#3795) and absorbance read at 450 nm with background subtraction reading at 650 nm on an Biotek Epoch plate reader (BioTek Inc., Winooski, VT, USA). .. Glutamine levels were measured in cell culture media using an immobilized enzyme electrode-based YSI 2950 Biochemistry Analyzer (YSI Life Sciences, Inc., Yellow Springs, OH, USA) and normalized to levels in control medium not exposed to cells but treated identically (to control for evaporation) and to cell number.

    Membrane:

    Article Title: Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer
    Article Snippet: Densitometry was performed using an Odyssey Infrared Imaging System and software (LI-COR Biosciences Inc., Lincoln, NE, USA); densitometry signals for glutamine synthetase, glutaminase, and glutamate dehydrogenase were normalized to actin densitometry levels. .. Semipermeable transwell membrane inserts (0.4 μm, Corning Costar Inc., Tewksbury, MA, USA) containing 15,000 3T3L1 preadipocytes or mature adipocytes were placed above 2000 PanIN/PDAC cells in 24-well culture plates in 500 μl DMEM, 0.5%FCS with glucose/glutamine concentrations as indicated, cultured 72 h, transwells removed, and 50 μl XTT reagent (Biotium Inc., Hayward, CA, USA) added, cells incubated overnight, then 100 μl of media was removed and added to a 96-well assay plate (Corning Costar Inc., Tewksbury MA, USA, Cat#3795) and absorbance read at 450 nm with background subtraction reading at 650 nm on an Biotek Epoch plate reader (BioTek Inc., Winooski, VT, USA). .. Glutamine levels were measured in cell culture media using an immobilized enzyme electrode-based YSI 2950 Biochemistry Analyzer (YSI Life Sciences, Inc., Yellow Springs, OH, USA) and normalized to levels in control medium not exposed to cells but treated identically (to control for evaporation) and to cell number.

    Cell Culture:

    Article Title: Adipocytes promote pancreatic cancer cell proliferation via glutamine transfer
    Article Snippet: Densitometry was performed using an Odyssey Infrared Imaging System and software (LI-COR Biosciences Inc., Lincoln, NE, USA); densitometry signals for glutamine synthetase, glutaminase, and glutamate dehydrogenase were normalized to actin densitometry levels. .. Semipermeable transwell membrane inserts (0.4 μm, Corning Costar Inc., Tewksbury, MA, USA) containing 15,000 3T3L1 preadipocytes or mature adipocytes were placed above 2000 PanIN/PDAC cells in 24-well culture plates in 500 μl DMEM, 0.5%FCS with glucose/glutamine concentrations as indicated, cultured 72 h, transwells removed, and 50 μl XTT reagent (Biotium Inc., Hayward, CA, USA) added, cells incubated overnight, then 100 μl of media was removed and added to a 96-well assay plate (Corning Costar Inc., Tewksbury MA, USA, Cat#3795) and absorbance read at 450 nm with background subtraction reading at 650 nm on an Biotek Epoch plate reader (BioTek Inc., Winooski, VT, USA). .. Glutamine levels were measured in cell culture media using an immobilized enzyme electrode-based YSI 2950 Biochemistry Analyzer (YSI Life Sciences, Inc., Yellow Springs, OH, USA) and normalized to levels in control medium not exposed to cells but treated identically (to control for evaporation) and to cell number.



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